RAD1 Polyclonal antibody

RAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11726-2-AP

Synonyms

REC1, Rad1-like DNA damage checkpoint protein, HRAD1

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Validation Data Gallery

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  WB analysis using 11726-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11726-2-AP (RAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11726-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11726-2-AP (RAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11726-2-AP

  IP result of anti-RAD1 (IP:11726-2-AP, 4ug; Detection:11726-2-AP 1:2000) with HeLa cells lysate 1200 ug.

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  IHC staining of human lung using 11726-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11726-2-AP (RAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 11726-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 11726-2-AP (RAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11726-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11726-2-AP (RAD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse spleen tissue, K-562 cells, L02 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

11726-2-AP targets RAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat, rainbow trout
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD1 fusion protein Ag2316
• Full Name: RAD1 homolog (S. pombe)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC006837
• Gene Symbol: RAD1
• Gene ID (NCBI): 5810
• RRID: AB_2246457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD1, cell cycle checkpoint protein, is a component of a heterotrimeric cell cycle checkpoint 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. RAD1, HUS1 and RAD9 are central components of a DNA damage-responsive protein complex. Testis-specific disruption of RAD1 in mice resulted in defective double-strand break repair, depletion of germ cells and infertility (PMID: 35133274). RAD1 has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	J Mol Cell Biol; SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.; Authors - Xiaoli Xu
human	WB	Am J Physiol Cell Physiol; Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.; Authors - Rongyi Shi
rainbow trout	WB	Comp Biochem Physiol C Toxicol Pharmacol; The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.; Authors - Bozdarov Johny J
rat	WB	Mol Med; Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia; Authors - Xin Tong