RAD1 Polyclonal antibody

RAD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 11726-2-AP


HRAD1, RAD1, RAD1 homolog (S. pombe), REC1

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

11726-2-AP targets RAD1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, rainbow trout
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD1 fusion protein Ag2316
Full Name RAD1 homolog (S. pombe)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC006837
Gene Symbol RAD1
Gene ID (NCBI) 5810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD1, cell cycle checkpoint protein, is a component of a heterotrimeric cell cycle checkpoint 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. RAD1, HUS1 and RAD9 are central components of a DNA damage-responsive protein complex. Testis-specific disruption of RAD1 in mice resulted in defective double-strand break repair, depletion of germ cells and infertility (PMID: 35133274). RAD1 has 3 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for RAD1 antibody 11726-2-APDownload protocol
IHC protocol for RAD1 antibody 11726-2-APDownload protocol
IF protocol for RAD1 antibody 11726-2-APDownload protocol
IP protocol for RAD1 antibody 11726-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu

Am J Physiol Cell Physiol

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

Authors - Rongyi Shi
rainbow troutWB

Comp Biochem Physiol C Toxicol Pharmacol

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

Authors - Bozdarov Johny J

Mol Med

Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia

Authors - Xin Tong