RAD51 Monoclonal antibody

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• RAD51 Monoclonal antibody (67024-1-Ig)

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• KD/KO Validated

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67024-1-Ig

Clone No.4A12B8

Publications(25)

Reviews (2)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

4A12B8, BRCC5, HRAD51, HsRad51, HsT16930

• 

  WB analysis using 67024-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 67024-1-Ig

  WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of 4T1 using 67024-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 67024-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 67024-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67024-1-Ig targets RAD51 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7410

  Product name: Recombinant human RAD51 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-242 aa of BC001459

  Sequence: MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

• Full Name: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001459
• Gene Symbol: RAD51
• Gene ID (NCBI): 5888
• RRID: AB_2882339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Nanobiotechnology; Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles.; Authors - Ruijie Qian
human,rat	WB,IF	Int J Surg; Multi-target modulation of the homologous recombination pathway by resveratrol promotes DNA damage repair in POI: integrated network pharmacology, molecular dynamics simulation, and experimental validation.; Authors - Zhaoyang Yu
human	WB	Signal Transduct Target Ther; Irinotecan alleviates chemoresistance to anthracyclines through the inhibition of AARS1-mediated BLM lactylation and homologous recombination repair; Authors - Xinyuan Li
human	WB	Discov Oncol; Multi-omics integration of TPX2 in prognostic prediction in resectable hepatocellular carcinoma; Authors - Haoran Zhao
		Iran J Biotechnol; Targeting TPX2 to Enhance Metastasis Control and Radiosensitivity in Esophageal Squamous Cell Carcinoma Through PI3K/AKT Pathway Modulation.; Authors - Fang Meng
human	WB	Nat Cell Biol; Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection; Authors - Yixin Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Md Asad (Verified Customer) (02-24-2026)

Very convincing result! I used this antibody (anti RAD51) to examine the activation homologous recombination DNA repair pathway in response to chemotherapy treatment.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MDA-MB-231

Juri (Verified Customer) (01-02-2024)

We have tested in immunocytochemistry, but did not work. Kindly, proteintech offered a replacement.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: Cell