RAI1 Polyclonal antibody

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  WB analysis of HeLa using 17084-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17084-1-AP (RAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17084-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17084-1-AP (RAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RAI1 Polyclonal Antibody for WB, ELISA

Cat No. 17084-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIAA1820, retinoic acid induced 1, Retinoic acid-induced protein 1, SMCR, SMS

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17084-1-AP targets RAI1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9046

  Product name: Recombinant human RAI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC021209

  Sequence: MQSFRERCGFHGKQQNYQQTSQETSRLENYRQPSQAGLSCDRQRLLAKDYYNPQPYPSYEGGAGTPSGTAAAVAADKYHRGSKALPTQQGLQGRPAFPGYGVQDSSPYPGRYAGEESLQAWGAPQPPPPQPQPLPAGVAKYDENLMKKTAVPPSRQYAEQGAQVPFRTHSLHVQQPPPPQQPLAYPKLQRQKLQNDIASPLPFPQGTHFPQHSQSFPTSSTYSSSVQGGGQGAHSYKSCTAPTAQPHDRPLTASSSLAPGQRVQNLHAYQSGRLSYDQQQQQQQQQQQQQQALQSRHHAQETLHYQNLAKYQHYGQQGQGYCQPDAAVRTPEQYYQTFSPSSSHSPARSV

• Full Name: retinoic acid induced 1
• Calculated Molecular Weight: 1906aa,203 kDa; 966aa,104 kDa
• Observed Molecular Weight: 250-260 kDa
• GenBank Accession Number: BC021209
• Gene Symbol: RAI1
• Gene ID (NCBI): 10743
• RRID: AB_2284824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z5J4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RAI1 (Retinoic Acid Induced 1) gene is located on the short arm of chromosome 17. The RAI1 protein it encodes is a crucial transcriptional regulator, playing a central role in processes such as neurodevelopment, sleep regulation, circadian rhythms, and energy metabolism. This protein contains a chromatin domain that binds to specific DNA sequences and recruits other transcriptional co-factors to activate or repress the expression of downstream target genes. The RAI1 gene is extremely sensitive to haploinsufficiency, and its dysfunction is closely linked to several severe neurodevelopmental disorders. For instance, RAI1 haploinsufficiency causes Smith-Magenis syndrome, while duplication of the RAI1 gene is associated with Potocki-Lupski syndrome. Consequently, the RAI1 protein is a key molecule for understanding brain function and behavioral regulation, and a major focus of research for related genetic syndromes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Gene; A de novo mutation (p.S1419F) of Retinoic Acid Induced 1 is responsible for a patient with Smith-Magenis syndrome exhibiting schizophrenia; Authors - Rong Yu