RBAP48 Polyclonal antibody

RBAP48 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14557-1-AP

Synonyms



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, mouse thymus tissue
Positive IHC detected inhuman ovary tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14557-1-AP targets RBAP48 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBAP48 fusion protein Ag6110
Full Name retinoblastoma binding protein 4
Calculated molecular weight 48 kDa
Observed molecular weight 40 kDa, 48-53 kDa
GenBank accession numberBC053904
Gene symbol RBBP4
Gene ID (NCBI) 5928
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBAP48 antibody 14557-1-APDownload protocol
IHC protocol for RBAP48 antibody 14557-1-APDownload protocol
IF protocol for RBAP48 antibody 14557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols