RBAP48 Polyclonal antibody

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• RBAP48 Polyclonal antibody (20364-1-AP)

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• KD/KO Validated

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20364-1-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RBBP4, NURF55, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48

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  WB analysis using 20364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 20364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 20364-1-AP

  WB result of RBAP48 antibody (20364-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 20364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 20364-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 20364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 20364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 20364-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 20364-1-AP

  IP result of anti-RBAP48 (IP:20364-1-AP, 4ug; Detection:20364-1-AP 1:1000) with HeLa cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 20364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (20364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20364-1-AP targets RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: retinoblastoma binding protein 4
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: NM_005610
• Gene Symbol: RBBP4
• Gene ID (NCBI): 5928
• RRID: AB_10694151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09028
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB, IHC	J Clin Invest; ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.; Authors - Pingping Zhu
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
	WB	Carcinogenesis; circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.; Authors - Wei Zhan
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Chembiochem; Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.; Authors - Fang-Fang Zhuo ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Katherine (Verified Customer) (09-25-2023)

Nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T