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  • KD/KO Validated

RBAP48 Polyclonal antibody

RBAP48 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 20364-1-AP


CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

Tested Applications

Positive WB detected inA431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20364-1-AP targets RBAP48 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name retinoblastoma binding protein 4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberNM_005610
Gene Symbol RBBP4
Gene ID (NCBI) 5928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.


Product Specific Protocols
WB protocol for RBAP48 antibody 20364-1-APDownload protocol
IHC protocol for RBAP48 antibody 20364-1-APDownload protocol
IF protocol for RBAP48 antibody 20364-1-APDownload protocol
IP protocol for RBAP48 antibody 20364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
humanWB, IHC

J Clin Invest

ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.

Authors - Pingping Zhu

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila


circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.

Authors - Wei Zhan

Int J Biol Sci

Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.

Authors - Shang Zhi
  • KD Validated


Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.

Authors - Fang-Fang Zhuo
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Katherine (Verified Customer) (09-25-2023)

Nice band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T