RBAP48 Polyclonal antibody

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  WB analysis using 20364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20364-1-AP

  WB result of RBAP48 antibody (20364-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

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  WB analysis of HepG2 using 20364-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20364-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20364-1-AP

  IP result of anti-RBAP48 (IP:20364-1-AP, 4ug; Detection:20364-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (20364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20364-1-AP

Publications(14)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RBBP4, NURF55, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48

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Tested Applications

• Positive WB detected in: A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Product Information

20364-1-AP targets RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: retinoblastoma binding protein 4
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: NM_005610
• Gene Symbol: RBBP4
• Gene ID (NCBI): 5928
• RRID: AB_10694151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09028
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB, IHC	J Clin Invest; ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.; Authors - Pingping Zhu
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
	WB	Carcinogenesis; circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.; Authors - Wei Zhan
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Chembiochem; Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.; Authors - Fang-Fang Zhuo ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

Nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T