RBAP48 Polyclonal antibody

RBAP48 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20364-1-AP

Synonyms

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human brain tissue, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20364-1-AP targets RBAP48 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name retinoblastoma binding protein 4
Calculated molecular weight 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberNM_005610
Gene symbol RBBP4
Gene ID (NCBI) 5928
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Protocols

Product Specific Protocols
WB protocol for RBAP48 antibody 20364-1-APDownload protocol
IHC protocol for RBAP48 antibody 20364-1-APDownload protocol
IF protocol for RBAP48 antibody 20364-1-APDownload protocol
IP protocol for RBAP48 antibody 20364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

PFA ependymoma-associated protein EZHIP inhibits PRC2 activity through a H3 K27M-like mechanism.

Authors - Siddhant U Jain
WB

Carcinogenesis

circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.

Authors - Wei Zhan
humanWB, IHC

J Clin Invest

ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.

Authors - Pingping Zhu
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu