Tested Applications
| Positive WB detected in | A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20364-1-AP targets RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | retinoblastoma binding protein 4 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | NM_005610 |
| Gene Symbol | RBBP4 |
| Gene ID (NCBI) | 5928 |
| RRID | AB_10694151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q09028 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBAP48 antibody 20364-1-AP | Download protocol |
| IHC protocol for RBAP48 antibody 20364-1-AP | Download protocol |
| IP protocol for RBAP48 antibody 20364-1-AP | Download protocol |
| WB protocol for RBAP48 antibody 20364-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RBBP4 antibody (Cat# 20364-1-AP) has 17 citations published in journals including Nature Cell Biology, Nature Communications, Signal Transduction and Targeted Therapy, Nucleic Acids Research, Journal of Clinical Investigation, Oncogene, Journal of Biological Chemistry, and others. Associated research fields encompass epigenetic chromatin regulation (PRC2/NuRD complexes), cancer biology (breast, colorectal, lung, B-cell lymphoma), stem cell biology, lipid metabolism, and DNA damage repair.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. | ||
Nat Commun PFA ependymoma-associated protein EZHIP inhibits PRC2 activity through a H3 K27M-like mechanism. | ||
Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development | ||
J Clin Invest ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells. |
Reviews
What customers say
This product has received one review from Katherine Chen (September 2023), who rated it 5 out of 5 stars. She used the antibody for Western Blot on 293T cells at a 1:2000 dilution and reported a clean, specific band, describing the result simply as "nice band." Overall, the feedback is positive, indicating good performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katherine (Verified Customer) (09-25-2023) | Nice band
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