Validation Data Gallery
|Positive WB detected in||A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervical cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20364-1-AP targets RBAP48 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||retinoblastoma binding protein 4|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48-50 kDa|
|GenBank accession number||NM_005610|
|Gene ID (NCBI)||5928|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.
|Product Specific Protocols|
|WB protocol for RBAP48 antibody 20364-1-AP||Download protocol|
|IHC protocol for RBAP48 antibody 20364-1-AP||Download protocol|
|IF protocol for RBAP48 antibody 20364-1-AP||Download protocol|
|IP protocol for RBAP48 antibody 20364-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Katherine (Verified Customer) (09-25-2023)