RBM20 Recombinant antibody

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  WB analysis using 83363-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 83363-1-RR

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83363-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

RBM20 Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83363-1-RR

Clone No.240345B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

RNA binding protein, RNA binding motif protein 20, 240345B7

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Tested Applications

• Positive WB detected in: HEK-293T cells, K-562 cells, TT cells, A549 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83363-1-RR targets RBM20 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBM20 fusion protein Ag34322
• Full Name: RNA binding motif protein 20
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_001134363
• Gene Symbol: RBM20
• Gene ID (NCBI): 282996
• RRID: AB_3671017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T481
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).