RBM20 Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 83363-1-RR (same clone as 83363-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00379-4

  Sandwich ELISA standard curve of MP00379-4, Human RBM20 Recombinant Matched Antibody Pair - PBS only. 83363-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34322. 83363-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00379-4

  The mean RBM20 concentration was determined to be 1.8 ng/mL in A549 cell extract based on a 2.0 mg/mL extract load.

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  FC experiment of K-562 using 83363-1-RR (same clone as 83363-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00379-1

  Cytometric bead array standard curve of MP00379-1, RBM20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83363-1-PBS. Detection antibody: 83363-2-PBS. Standard: Ag34322. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00379-2

  Cytometric bead array standard curve of MP00379-2, RBM20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83363-3-PBS. Detection antibody: 83363-1-PBS. Standard: Ag34322. Range: 1.25-80 ng/mL.

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  Affinity and Kinetic Characterization of 83363-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

RBM20 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83363-1-PBS

Clone No.240345B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

RNA binding protein, RNA binding motif protein 20, 240345B7

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Product Information

83363-1-PBS targets RBM20 as part of a matched antibody pair:

MP00379-1: 83363-1-PBS capture and 83363-2-PBS detection (validated in Cytometric bead array)

MP00379-2: 83363-3-PBS capture and 83363-1-PBS detection (validated in Cytometric bead array)

MP00379-4: 83363-2-PBS capture and 83363-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBM20 fusion protein Ag34322
• Full Name: RNA binding motif protein 20
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_001134363
• Gene Symbol: RBM20
• Gene ID (NCBI): 282996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T481
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).