RBM24 Polyclonal antibody

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• RBM24 Polyclonal antibody (18178-1-AP)

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• KD/KO Validated

RBM24 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 18178-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, RIP, ELISA

Synonyms

RNA binding motif protein 24, RNA binding protein 24, RNA-binding motif protein 24, RNA-binding protein 24, RNA-binding region-containing protein 6

• 

  WB analysis using 18178-1-AP

  mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18178-1-AP (RBM24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18178-1-AP (RBM24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18178-1-AP targets RBM24 in WB, IHC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12871

  Product name: Recombinant human RBM24 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-191 aa of BC104808

  Sequence: MYVCLCVSVAKVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRIMQPGFAFGVQQLHPALIQRPFGIPAHYVYPQAFVQPGVVIPHVQPTAAAASTTPYIDYTGAAYAQYSAAAAAAAAAAAYDQYPYAASPAAAGYVTAGGYGYAVQQPITAAAPGTAAAAAAAAAAAAAFGQYQPQQLQTDRMQ

• Full Name: RNA binding motif protein 24
• Calculated Molecular Weight: 191 aa, 20 kDa
• Observed Molecular Weight: 18-25 kDa
• GenBank Accession Number: BC104808
• Gene Symbol: RBM24
• Gene ID (NCBI): 221662
• RRID: AB_2878513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BX46
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).

Publications

What published studies show

The anti-RBM24 antibody (Cat# 18178-1-AP) has 14 citations spanning journals including Cell Death & Differentiation, PNAS, eLife, Development, Scientific Reports, and International Journal of Biological Macromolecules. Research fields cover cancer biology (ovarian, lung, colorectal, thyroid carcinoma), cochlear hair cell development and hearing, myogenic differentiation, RNA splicing regulation, cuproptosis, and neurovascular disease. Applications include WB, IF, IHC, and RIP in human and mouse samples.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Differ; Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer; Authors - Tong Xu
mouse	WB	Proc Natl Acad Sci U S A; Alternative splicing of the Rbm24 gene is essential for cochlear hair cell stereocilia integrity and hearing function in mice; Authors - Chunjiao Sun ; KO Validated
mouse	WB,IHC	Int J Biol Macromol; Rbm24 modulates neuronal RNA splicing to restrict cognitive dysfunction; Authors - Dongbo Yao
human	RIP,IHC	J Mol Med (Berl); Mechanism of METTL14-mediated m^6A modification in non-small cell lung cancer cell resistance to cisplatin; Authors - Shulei Gong
mouse	IF	Sci Rep; Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.; Authors - Raphaëlle Grifone ; KD Validated
mouse	IF	J Cell Physiol; RBM24 is required for mouse hair cell development through regulating pre-mRNA alternative splicing and mRNA stability; Authors - Yanfei Wang