Tested Applications
| Positive WB detected in | mouse thymus tissue, rat thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13225-1-AP targets RCBTB2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3975 Product name: Recombinant human RCBTB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-551 aa of BC029052 Sequence: TIACGQMCCMAVVDTGEVYVWGYNGNGQLGLGNSGNQPTPCRVAALQGIRVQRVACGYAHTLVLTDEGQVYAWGANSYGQLGTGNKSNQSYPTPVTVEKDRIIEIAACHSTHTSAAKTQGGHVYMWGQCRGQSVILPHLTHFSCTDDVFACFATPAVTWRLLSVEPDDHLTVAESLKREFDNPDTADLKFLVDGKYIYAHKVLLKIRCEHFRSSLEDNEDDIVEMSEFSYPVYRAFLEYLYTDSISLSPEEAVGLLDLATFYRENRLKKLCQQTIKQGICEENAIALLSAAVKYDAQDLEEFCFRFCINHLTVVTQTSGFAEMDHDLLKNFISKASRVGAFKN Predict reactive species |
| Full Name | regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 |
| Calculated Molecular Weight | 551 aa, 60 kDa |
| Observed Molecular Weight | 58-60 kDa |
| GenBank Accession Number | BC029052 |
| Gene Symbol | RCBTB2 |
| Gene ID (NCBI) | 1102 |
| RRID | AB_2179570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95199 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RCBTB2 antibody 13225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RCBTB2 Polyclonal antibody (Cat# 13225-1-AP) has 4 total citations. These appear in Nature Communications, Biomedical and Environmental Sciences, Toxicology and Applied Pharmacology, and American Journal of Cancer Research. The associated research fields include spermatogenesis and reproductive biology, ovarian cancer proliferation and invasion, hepatoma cell toxicity and proteomic profiling, and prostate cancer progression involving aggrephagy regulation.
| Species | Application | Title |
|---|---|---|
Biomed Environ Sci Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells. | ||
Toxicol Appl Pharmacol Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde. | ||
Am J Cancer Res The ubiquitin ligase RCBTB2 regulates aggrephagy and inhibits prostate cancer progression by targeting GPAA1 for degradation. |



