RCCD1 Polyclonal antibody, PBS Only

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  WB analysis using 24446-1-AP (same clone as 24446-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24446-1-AP (RCCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP (same clone as 24446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP (same clone as 24446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 24446-1-AP (same clone as 24446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 24446-1-AP (same clone as 24446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

RCCD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24446-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

RCC1 domain-containing protein 1, RCC1 domain containing 1

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Product Information

24446-1-PBS targets RCCD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCCD1 fusion protein Ag19685
• Full Name: RCC1 domain containing 1
• Calculated Molecular Weight: 376 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC140708
• Gene Symbol: RCCD1
• Gene ID (NCBI): 91433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NED2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.