RCCD1 Polyclonal antibody

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  WB analysis using 24446-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24446-1-AP (RCCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 24446-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 24446-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 40x lens).

RCCD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24446-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RCC1 domain-containing protein 1, RCC1 domain containing 1

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24446-1-AP targets RCCD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCCD1 fusion protein Ag19685
• Full Name: RCC1 domain containing 1
• Calculated Molecular Weight: 376 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC140708
• Gene Symbol: RCCD1
• Gene ID (NCBI): 91433
• RRID: AB_3085734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NED2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.