Recoverin Polyclonal antibody

Recoverin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10073-1-AP

Synonyms

Protein CAR, RCV1, RCVRN, recoverin



Tested Applications

Positive WB detected inmouse eye tissue, rat retina tissue, Y79 cells, rat eye tissue
Positive IF detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10073-1-AP targets Recoverin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recoverin fusion protein Ag0119
Full Name recoverin
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001720
Gene Symbol RCVRN
Gene ID (NCBI) 5957
RRIDAB_2178005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

Protocols

Product Specific Protocols
WB protocol for Recoverin antibody 10073-1-APDownload protocol
IF protocol for Recoverin antibody 10073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Neurosci

Differentiation of marrow stromal cells into photoreceptors in the rat eye.

Authors - Kicic Anthony A
humanIF

iScience

A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.

Authors - Suguru Yamasaki
humanIF

iScience

One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors.

Authors - Yuki Otsuka
mouseWB

iScience

Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease.

Authors - Jie Zhang
humanIF

Sci Rep

Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue.

Authors - Atsushi Kuwahara
mouseIF

Sci Rep

Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration.

Authors - Kenichi Makabe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ROs