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RDH10 Polyclonal antibody

RDH10 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14644-1-AP


RDH10, Retinol dehydrogenase 10, SDR16C4

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14644-1-AP targets RDH10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, insect
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH10 fusion protein Ag6237
Full Name retinol dehydrogenase 10 (all-trans)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC067131
Gene Symbol RDH10
Gene ID (NCBI) 157506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).


Product Specific Protocols
WB protocol for RDH10 antibody 14644-1-APDownload protocol
IHC protocol for RDH10 antibody 14644-1-APDownload protocol
IP protocol for RDH10 antibody 14644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue.

Authors - Atsushi Kuwahara

Proc Natl Acad Sci U S A

Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct.

Authors - Tadaaki Nakajima

Curr Biol

Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary Rootlets.

Authors - Chloe Potter

Cell Prolif

Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120

Authors - Jun Jing

Cell Rep

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Authors - Tony DeFalco

Stem Cell Reports

Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

Authors - Albert Lowe


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Claudio (Verified Customer) (06-29-2020)

Antibody did not show specific staining/signal. Antigen retrieval pH 9.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: PFA-fixed paraffin-embedded mouse sections

Daniel (Verified Customer) (02-12-2019)

Validated with CRISPR/Cas9 knockout cell lines

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO)
RDH10 Antibody Western Blot, validation (1:1000 dilution) in Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO) (Cat no:14644-1-AP)