Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse kidney tissue, rat bladder tissue, human placenta tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14644-1-AP targets RDH10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, insect |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6237 Product name: Recombinant human RDH10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-293 aa of BC067131 Sequence: VRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINTQSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVSVLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDYCASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAILTDQPMICTPRLM Predict reactive species |
| Full Name | retinol dehydrogenase 10 (all-trans) |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC067131 |
| Gene Symbol | RDH10 |
| Gene ID (NCBI) | 157506 |
| RRID | AB_2177866 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RDH10 antibody 14644-1-AP | Download protocol |
| IP protocol for RDH10 antibody 14644-1-AP | Download protocol |
| WB protocol for RDH10 antibody 14644-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RDH10 (retinol dehydrogenase 10) antibody, catalog number 14644-1-AP, has 27 citations. These appear in journals including Nature Communications, PNAS, Developmental Cell, Current Biology, Cell Reports, the Journal of Biological Chemistry, FASEB Journal, iScience, and Scientific Reports. Research fields span retinoic acid metabolism and signaling, retinal/vision biology, development, cancer, neuroscience, reproductive biology, and metabolic disease (diabetes, liver disease). Applications are mainly WB, IF, and IHC in human, mouse, and rat.
| Species | Application | Title |
|---|---|---|
Nat Commun Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. | ||
Cell Commun Signal In vivo CRISPR screening identifies POU3F3 as a novel regulator of ferroptosis resistance in hepatocellular carcinoma via retinoic acid signaling | ||
Proc Natl Acad Sci U S A Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct. | ||
Dev Cell 3D reconstruction of the mouse cochlea from scRNA-seq data suggests morphogen-based principles in apex-to-base specification | ||
Reviews
What customers say
Two reviews were submitted for this antibody. One user reported successful Western Blot validation at 1:1000 dilution in embryonic stem cells, confirming specificity through CRISPR/Cas9 knockout comparisons across wild-type, heterozygote, and homozygote lines. Another user reported no specific staining in immunofluorescence on PFA-fixed paraffin-embedded mouse sections at 1:50 dilution with pH 9 antigen retrieval. Overall, the antibody performs well for WB with knockout validation, but IF results on fixed tissue sections may require optimization.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Claudio (Verified Customer) (06-29-2020) | Antibody did not show specific staining/signal. Antigen retrieval pH 9.
|
Daniel (Verified Customer) (02-12-2019) | Validated with CRISPR/Cas9 knockout cell lines
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