|Positive WB detected in||HepG2 cells, A549 cells, mouse liver tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14644-1-AP targets RDH10 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RDH10 fusion protein Ag6237|
|Full Name||retinol dehydrogenase 10 (all-trans)|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC067131|
|Gene ID (NCBI)||157506|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
J Alzheimers Dis
Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes.
Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain.
J Biol Chem
Changes in retinoid metabolism and signaling associated with metabolic remodeling during fasting and in type I diabetes.
J Biol Chem
The Antagonistically Bifunctional Retinoid Oxidoreductase Complex Is Required for Maintenance of All-trans-retinoic Acid Homeostasis.
Front Cell Dev Biol
Dietary Vitamin A Impacts Refractory Telogen.
The role of retinol dehydrogenase 10 in the cone visual cycle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Claudio (Verified Customer) (06-29-2020)
Antibody did not show specific staining/signal. Antigen retrieval pH 9.
Daniel (Verified Customer) (02-12-2019)
Validated with CRISPR/Cas9 knockout cell lines