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  • KD/KO Validated

RDH10 Polyclonal antibody

RDH10 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14644-1-AP

Synonyms

RDH10, Retinol dehydrogenase 10, SDR16C4



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14644-1-AP targets RDH10 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH10 fusion protein Ag6237
Full Name retinol dehydrogenase 10 (all-trans)
Calculated molecular weight 38 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC067131
Gene symbol RDH10
Gene ID (NCBI) 157506
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).

Protocols

Product Specific Protocols
WB protocol for RDH10 antibody 14644-1-APDownload protocol
IHC protocol for RDH10 antibody 14644-1-APDownload protocol
IP protocol for RDH10 antibody 14644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Alzheimers Dis

Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes.

Authors - Thabat Khatib
mouseIHC

PLoS One

Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain.

Authors - Tochitani Shiro S
mouseWB

J Biol Chem

Changes in retinoid metabolism and signaling associated with metabolic remodeling during fasting and in type I diabetes.

Authors - Alla V Klyuyeva
humanWB

J Biol Chem

The Antagonistically Bifunctional Retinoid Oxidoreductase Complex Is Required for Maintenance of All-trans-retinoic Acid Homeostasis.

Authors - Olga V Belyaeva
mouseIHC

Front Cell Dev Biol

Dietary Vitamin A Impacts Refractory Telogen.

Authors - Liye Suo
mouseWB

Sci Rep

The role of retinol dehydrogenase 10 in the cone visual cycle.

Authors - Yunlu Xue
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudio (Verified Customer) (06-29-2020)

Antibody did not show specific staining/signal. Antigen retrieval pH 9.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: PFA-fixed paraffin-embedded mouse sections
FH

Daniel (Verified Customer) (02-12-2019)

Validated with CRISPR/Cas9 knockout cell lines

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO)
RDH10 Antibody Western Blot, validation (1:1000 dilution) in Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO) (Cat no:14644-1-AP)