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RDH10 Polyclonal antibody
RDH10 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14644-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 9 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
Product Information
14644-1-AP targets RDH10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, insect |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RDH10 fusion protein Ag6237 |
Full Name | retinol dehydrogenase 10 (all-trans) |
Calculated molecular weight | 38 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC067131 |
Gene symbol | RDH10 |
Gene ID (NCBI) | 157506 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
Protocols
Product Specific Protocols | |
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WB protocol for RDH10 antibody 14644-1-AP | Download protocol |
IHC protocol for RDH10 antibody 14644-1-AP | Download protocol |
IP protocol for RDH10 antibody 14644-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. | ||
Proc Natl Acad Sci U S A Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct. | ||
Stem Cell Reports Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Claudio (Verified Customer) (06-29-2020) | Antibody did not show specific staining/signal. Antigen retrieval pH 9.
|
FH Daniel (Verified Customer) (02-12-2019) | Validated with CRISPR/Cas9 knockout cell lines
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