RDH10 Polyclonal antibody

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  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14644-1-AP

  IP result of anti-RDH10 (IP:14644-1-AP, 4ug; Detection:14644-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RDH10 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14644-1-AP

Publications(22)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RDH10, Retinol dehydrogenase 10, SDR16C4

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

14644-1-AP targets RDH10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, insect
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6237

  Product name: Recombinant human RDH10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-293 aa of BC067131

  Sequence: VRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINTQSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVSVLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDYCASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAILTDQPMICTPRLM

• Full Name: retinol dehydrogenase 10 (all-trans)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC067131
• Gene Symbol: RDH10
• Gene ID (NCBI): 157506
• RRID: AB_2177866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZV5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue.; Authors - Atsushi Kuwahara
mouse	WB	Proc Natl Acad Sci U S A; Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct.; Authors - Tadaaki Nakajima
mouse		Curr Biol; Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary Rootlets.; Authors - Chloe Potter
mouse	WB	Cell Prolif; Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120; Authors - Jun Jing
mouse	IF	Cell Rep; Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.; Authors - Tony DeFalco
human	IF	Stem Cell Reports; Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.; Authors - Albert Lowe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Claudio (Verified Customer) (06-29-2020)

Antibody did not show specific staining/signal. Antigen retrieval pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PFA-fixed paraffin-embedded mouse sections

FH

Daniel (Verified Customer) (02-12-2019)

Validated with CRISPR/Cas9 knockout cell lines

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO)

RDH10 Antibody Western Blot, validation (1:1000 dilution) in Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO) (Cat no:14644-1-AP)