|Positive WB detected in||HepG2 cells, A549 cells, mouse liver tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14644-1-AP targets RDH10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, insect, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RDH10 fusion protein Ag6237|
|Full Name||retinol dehydrogenase 10 (all-trans)|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC067131|
|Gene ID (NCBI)||157506|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
J Alzheimers Dis
Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes.
Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain.
Characterization of subunit interactions in the hetero-oligomeric retinoid oxidoreductase complex
J Biol Chem
Changes in retinoid metabolism and signaling associated with metabolic remodeling during fasting and in type I diabetes.
J Biol Chem
The Antagonistically Bifunctional Retinoid Oxidoreductase Complex Is Required for Maintenance of All-trans-retinoic Acid Homeostasis.
Front Cell Dev Biol
Dietary Vitamin A Impacts Refractory Telogen.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Claudio (Verified Customer) (06-29-2020)
Antibody did not show specific staining/signal. Antigen retrieval pH 9.
Daniel (Verified Customer) (02-12-2019)
Validated with CRISPR/Cas9 knockout cell lines