RGMA Polyclonal antibody

• Featured Product
• KD/KO Validated

RGMA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12387-1-AP

Synonyms

Repulsive guidance molecule A, RGM, RGM domain family member A, RGM domain family, member A, RGMA

---

Validation Data Gallery

• 

  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12387-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12387-1-AP

  IP result of anti-RGMA (IP:12387-1-AP, 4ug; Detection:12387-1-AP 1:400) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, mouse testis tissue, HeLa cells, MOLT-4 cells, U2OS cells, Caco-2 cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

12387-1-AP targets RGMA in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGMA fusion protein Ag3058
• Full Name: RGM domain family, member A
• Calculated Molecular Weight: 450 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC015886
• Gene Symbol: RGMA
• Gene ID (NCBI): 56963
• RRID: AB_2179366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGMA, also named as RGM, is a member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. RGMA is a molecular target for neuroprotection in retinal pathologies(PMID: 20457227). the frequent genetic and epigenetic inactivation of RGMA in CRCs and adenomas along with its in vitro function collectively support its role as a tumor suppressor in colon cells(PMID:19303019 ). There're some different MW in WB detection, 30 kDa mature form, 47-50 kDa isoforms and 60 kDa Glycosylated form.

Publications

Species	Application	Title
rat	WB	Brain Res Bull; Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.; Authors - Min Li ; KD Validated
mouse	CoIP	Sci Adv; RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS; Authors - Mikito Shimizu