RHEB Monoclonal antibody

RHEB Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2F9H8

Cat no : 66714-1-Ig

Synonyms

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2



Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells, DU 145 cells, mouse brain tissue
Positive IF detected inhuman pancreas cancer tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66714-1-Ig targets RHEB in WB, IF,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHEB fusion protein Ag8887
Full Name Ras homolog enriched in brain
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC016155
Gene symbol RHEB
Gene ID (NCBI) 6009
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Protocols

Product Specific Protocols
WB protocol for RHEB antibody 66714-1-IgDownload protocol
IF protocol for RHEB antibody 66714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols