RILP Polyclonal antibody

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  WB analysis of HEK-293 using 13574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13574-1-AP (RILP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13574-1-AP (RILP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13574-1-AP (RILP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13574-1-AP (RILP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13574-1-AP (RILP Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 13574-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RILP antibody (13574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

RILP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13574-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA, PLA

Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

13574-1-AP targets RILP in WB, IHC, IF/ICC, ELISA, PLA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RILP fusion protein Ag4472
• Full Name: Rab interacting lysosomal protein
• Calculated Molecular Weight: 401 aa, 41 kDa
• Observed Molecular Weight: 41-45 kDa
• GenBank Accession Number: BC031621
• Gene Symbol: RILP
• Gene ID (NCBI): 83547
• RRID: AB_2877959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NA2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
human	PLA	J Extracell Vesicles; Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles.; Authors - Xuefeng Fei
human	WB	J Exp Clin Cancer Res; Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.; Authors - Minghui Chang
human	WB	J Biol Chem; A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.; Authors - Mai E Oguchi ; KD Validated
	WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu
human	WB	Signal Transduct Target Ther; Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.; Authors - Wenchang Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (03-22-2021)

There is weak band between 35 and 50 kD. Seems working with WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK lysate

RILP Antibody Western Blot validation (1:500 dilution) in HEK lysate (Cat no:13574-1-AP)