|Positive WB detected in
|HEK-293 cells, HepG2 cells
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:200-1:1000
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13574-1-AP targets RILP in WB, PLA, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|RILP fusion protein Ag4472
|Rab interacting lysosomal protein
|Calculated molecular weight
|401 aa, 41 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
J Extracell Vesicles
Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles.
J Exp Clin Cancer Res
Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.
J Biol Chem
A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.
Cell Death Dis
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Signal Transduct Target Ther
Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.
Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Q (Verified Customer) (03-22-2021)
There is weak band between 35 and 50 kD. Seems working with WB.