RIM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RIM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24576-1-AP

Synonyms

CORD7, KIAA0340, Rab 3 interacting molecule 1, Rab 3 interacting protein 2, RAB3IP2, RIM, RIM 1, RIM1, RIMS1

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Validation Data Gallery

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  WB analysis of mouse brain using 24576-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24576-1-AP (RIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 24576-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24576-1-AP (RIM1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 24576-1-AP

  IP Result of anti-RIM1 (IP:24576-1-AP, 4ug; Detection:24576-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 24576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24576-1-AP (RIM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24576-1-AP (RIM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

The immunogen of 24576-1-AP is RIM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIM1 fusion protein Ag20161
• Full Name: regulating synaptic membrane exocytosis 1
• Calculated molecular weight: 1692 aa, 189 kDa
• Observed molecular weight: 189 kDa
• GenBank accession number: BC152435
• Gene symbol: RIMS1
• Gene ID (NCBI): 22999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Neurochem Res; Abnormal Expression of FBXL20 in Refractory Epilepsy Patients and a Pilocarpine-Induced Rat Model.; Authors - Pengfei Fu
mouse	WB	Neuron; Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors; Authors - ; KO Validated
rat	WB	Neurochem Int; Effects of exercise-induced fatigue on the morphology of asymmetric synapse and synaptic protein levels in rat striatum.; Authors - Zhifeng Wang