RIM1 Polyclonal antibody

RIM1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24576-1-AP

Synonyms

CORD7, KIAA0340, Rab 3 interacting molecule 1, Rab 3 interacting protein 2, RAB3IP2, RIM, RIM 1, RIM1, RIMS1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24576-1-AP is RIM1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIM1 fusion protein Ag20161
Full Name regulating synaptic membrane exocytosis 1
Calculated molecular weight 1692 aa, 189 kDa
Observed molecular weight 189 kDa
GenBank accession numberBC152435
Gene symbol RIMS1
Gene ID (NCBI) 22999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RIM1 antibody 24576-1-APDownload protocol
IHC protocol for RIM1 antibody 24576-1-APDownload protocol
IP protocol for RIM1 antibody 24576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neurochem Int

Effects of exercise-induced fatigue on the morphology of asymmetric synapse and synaptic protein levels in rat striatum.

Authors - Zhifeng Wang
mouseWB

Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors

Authors -
  • KO Validated
humanWB

Neurochem Res

Abnormal Expression of FBXL20 in Refractory Epilepsy Patients and a Pilocarpine-Induced Rat Model.

Authors - Pengfei Fu