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RIPK1-Specific Polyclonal antibody

RIPK1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


mouse, rat and More (3)





Cat no : 17519-1-AP


Cell death protein RIP, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1, RIPK1-Specific

Tested Applications

Positive WB detected inmouse heart tissue, rat liver tissue, rat heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17519-1-AP targets RIPK1-Specific in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse, rat, yeast, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated Molecular Weight 76 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberNM_003804
Gene Symbol RIPK1
Gene ID (NCBI) 8737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).


Product Specific Protocols
WB protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
IHC protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan

Circ Res

AirnRegulates Igf2bp2 Translation in Cardiomyocytes.

Authors - Mohammed R Hosen

Nat Commun

Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis.

Authors - Jiefei Geng

Sci Total Environ

Evaluation of 6-PPD quinone toxicity on lung of male BALB/c mice by quantitative proteomics

Authors - Wenmiao He

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie

Environ Pollut

Polystyrene nanoplastics and cadmium co-exposure aggravated cardiomyocyte damage in mice by regulating PANoptosis pathway

Authors - Jiali Ye


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yuan (Verified Customer) (01-07-2020)

This RIPK-1 antibody was tried on the MCF7 tamoxifen resistant (5uM) cell line, the 74 kDa band was very faint, but the 45 kDa band is clear. In addition, some unspecific bands near 45 kDa showed up too.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7-TR5