RIPK1-Specific Polyclonal antibody
RIPK1-Specific Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (3)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
100
Cat no : 17519-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat liver tissue, rat heart tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 90 publications below |
| IHC | See 10 publications below |
| IF | See 13 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
Product Information
17519-1-AP targets RIPK1-Specific in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, yeast, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | NM_003804 |
| Gene Symbol | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_10642833 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIPK1-Specific antibody 17519-1-AP | Download protocol |
| IHC protocol for RIPK1-Specific antibody 17519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein. | ||
Nat Commun Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis. | ||
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
PLoS Biol The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53. | ||
Free Radic Biol Med TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yuan (Verified Customer) (01-07-2020) | This RIPK-1 antibody was tried on the MCF7 tamoxifen resistant (5uM) cell line, the 74 kDa band was very faint, but the 45 kDa band is clear. In addition, some unspecific bands near 45 kDa showed up too.
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