RIPK1-Specific Polyclonal antibody

RIPK1-Specific Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP

Conjugate

Unconjugated

Cat no : 17519-1-AP

Synonyms

Cell death protein RIP, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1



Tested Applications

Positive WB detected inNIH/3T3 cells, UV treated Hela, rat liver tissue, K-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

17519-1-AP targets RIPK1-Specific in WB, IP, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated molecular weight 76 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
Gene ID (NCBI) 8737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

Protocols

Product Specific Protocols
WB protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
IHC protocol for RIPK1-Specific antibody 17519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicology

A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation.

Authors - Ziqiao Yuan
humanWB

Cardiovasc Drugs Ther

PMID: 30675709

Authors - Jingyi Chen
mouseWB,IHC

Cell Death Differ

The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling.

Authors - Feng-Xia Guo
mouseWB

Toxins (Basel)

Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis.

Authors - Rui Wang
humanWB

Toxicol In Vitro

Gallic acid protects against ethanol-induced hepatocyte necroptosis via an NRF2-dependent mechanism.

Authors - Ying Zhou
mouseWB

Toxicol Appl Pharmacol

Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.

Authors - Yanling Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (01-07-2020)

This RIPK-1 antibody was tried on the MCF7 tamoxifen resistant (5uM) cell line, the 74 kDa band was very faint, but the 45 kDa band is clear. In addition, some unspecific bands near 45 kDa showed up too.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7-TR5