Validation Data Gallery
|Positive WB detected in||Jurkat cells, K-562 cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells, HL-60 cells, HT-29 cells|
|Positive IHC detected in||human small intestine tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U2OS cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Full Name||receptor (TNFRSF)-interacting serine-threonine kinase 1|
|Calculated molecular weight||76 kDa|
|Observed molecular weight||74 kDa, 45 kDa|
|GenBank accession number||NM_003804|
|Gene ID (NCBI)||8737|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
|Product Specific Protocols|
|WB protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig||Download protocol|
|IHC protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig||Download protocol|
|IF protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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