Phospho-RIPK1 (Ser161) Monoclonal antibody

Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (2)







Cat no : 66854-1-Ig


Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

Tested Applications

Positive WB detected inJurkat cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells
Positive IHC detected inhuman small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
Gene ID (NCBI) 8737
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).


Product Specific Protocols
WB protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
IHC protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
IF protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Mol Med

LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.

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Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.

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