Tested Applications
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-66854 targets Phospho-RIPK1 (Ser161) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 74 kDa, 45 kDa |
| GenBank Accession Number | NM_003804 |
| Gene Symbol | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_2935071 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13546 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Phospho-RIPK1 (Ser161) antibody CL647-66854 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-RIPK1 (Ser161) Monoclonal Antibody (1B2G1), catalog number CL647-66854, has accumulated 55 citations across high-impact journals including Nature Communications, Signal Transduction and Targeted Therapy, Biomaterials, Science Advances, Advanced Science, Cell Death & Disease, Cancer Letters, International Journal of Biological Sciences, Phytomedicine, Journal of Medicinal Chemistry, Clinical and Translational Medicine, and others. Associated research fields include necroptosis and PANoptosis, oncology, cardiovascular protection, neurology, renal injury, pulmonary inflammation, infectious diseases, and immunology.
| Species | Application | Title |
|---|---|---|
Nat Commun RIOK2 kinase regulates the translocation of the FADD-RIPK1-Caspase-8 complex to the ER and the cleavage of Gasdermin D to drive pyroptosis |
