Tested Applications
| Positive WB detected in | human urine sample |
| Positive IHC detected in | human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18172-1-AP targets RNASE2/EDN in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12834 Product name: Recombinant human RNASE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-161 aa of BC093678 Sequence: QFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTFLLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDNRDQRRDPPQYPVVPVHLDRII Predict reactive species |
| Full Name | ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) |
| Calculated Molecular Weight | 161 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC093678 |
| Gene Symbol | RNASE2 |
| Gene ID (NCBI) | 6036 |
| RRID | AB_3669293 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNASE2, also known as eosinophil-derived neurotoxin (EDN), is a member of the RNase A superfamily and is predominantly expressed in eosinophilic leukocytes, where it is stored in large quantities within specific cytoplasmic granules. Structurally, RNASE2 possesses a catalytic domain that confers ribonuclease activity, which enables its primary role in the host immune response against viral infections by directly degrading viral RNA, thereby inhibiting viral replication. Upon release from activated eosinophils, RNASE2 can signal through pattern recognition receptors such as Toll-like receptor 2 (TLR2) to activate dendritic cells and promote T-helper type 2 (Th2) immune responses. Elevated levels of RNASE2 are found in various inflammatory conditions, including asthma, allergic rhinitis, and parasitic infections, making it a key biomarker for eosinophil activation. Beyond its roles in immunity and inflammation, RNASE2 also exhibits neurotoxic activity, and has been implicated in wound healing and tissue remodeling processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RNASE2/EDN antibody 18172-1-AP | Download protocol |
| WB protocol for RNASE2/EDN antibody 18172-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Issues Mol Biol Identification of Paraptosis-Related Renal Cell Carcinoma Subtypes, Construction of a Prognostic Signature, and Determination of Tumor Microenvironment Landscape Using Bioinformatic Analysis and Experimental Verification. |
