RND3 Monoclonal antibody

• 

  WB analysis of fetal human brain using 66228-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66228-1-Ig (RND3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RND3 Monoclonal Antibody for WB, ELISA

Cat No. 66228-1-Ig

Clone No.3H8F9

Publications(4)

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF, ELISA

Synonyms

ARHE, memB, Protein MemB, Rho family GTPase 3, Rho8, RhoE, RND3

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Tested Applications

• Positive WB detected in: fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

66228-1-Ig targets RND3 in WB, IF, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18839

  Product name: Recombinant human RND3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-244 aa of BC012513

  Sequence: MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM

• Full Name: Rho family GTPase 3
• Calculated Molecular Weight: 244 aa, 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC012513
• Gene Symbol: RND3
• Gene ID (NCBI): 390
• RRID: AB_2881619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61587
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.; Authors - Yetao Xu
human	WB	Int J Mol Med; Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.; Authors - Zhongming Shao ; KO Validated
human	WB	Virology; Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection; Authors - Li Xia Yuan
human	IF	bioRxiv; The Hexosamine Biosynthetic Pathway alters the cytoskeleton to modulate cell proliferation and migration in metastatic prostate cancer; Authors - Rajina Shakya

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (12-10-2020)

This antibody is very good quality and can detect RND3 in both U937 and PBMC cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U937 & PBMC