RNF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 17810-1-AP

Synonyms

E3 ubiquitin ligase RNF4, Protein SNURF, RES4 26, ring finger protein 4, RNF4, SNURF

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Validation Data Gallery

• 

  WB analysis of PC-3 using 17810-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 17810-1-AP

  IP result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:300) with PC-3 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 17810-1-AP is RNF4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF4 fusion protein Ag12115
• Full Name: ring finger protein 4
• Calculated Molecular Weight: 190 aa, 21 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC031935
• Gene Symbol: RNF4
• Gene ID (NCBI): 6047
• RRID: AB_2878443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

Publications

Species	Application	Title
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB	Int J Mol Sci; The E3 Ubiquitin-Protein Ligase RNF4 Promotes TNF-α-Induced Cell Death Triggered by RIPK1.; Authors - Tatsuya Shimada
human	WB	ACS Chem Biol; Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications.; Authors - Carl C Ward
human	WB	J Biol Chem; Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control; Authors - Wei Wang ; KD Validated
mouse	WB,CoIP	Theranostics; CSF2RB overexpression promotes the protective effects of mesenchymal stromal cells against ischemic heart injury; Authors - Tingting Qi ; KD Validated
human	WB	Exp Cell Res; RNF4 promotes tumorigenesis, therapy resistance of cholangiocarcinoma and affects cell cycle by regulating the ubiquitination degradation of p27kip1 in the nucleus; Authors - Jie Huang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 cells