RNF6 Polyclonal antibody

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  WB analysis using 20437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20437-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 20437-1-AP

  IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IF Staining of SW480 using 20437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

• Featured Product
• KD/KO Validated

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 20437-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

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Tested Applications

• Positive WB detected in: DU 145 cells, mouse testis tissue, PC-3 cells, SKOV-3 cells, Sp2/0 cells
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

20437-1-AP targets RNF6 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14209

  Product name: Recombinant human RNF6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC034688

  Sequence: MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRTNPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSSNIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHVWENGARSNVTVRNTNQRLEP

• Full Name: ring finger protein (C3H2C3 type) 6
• Calculated Molecular Weight: 685 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC034688
• Gene Symbol: RNF6
• Gene ID (NCBI): 6049
• RRID: AB_10666853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF,ChIP	Oncogene; RING-finger protein 6 promotes colorectal tumorigenesis by transcriptionally activating SF3B2.; Authors - Hui Xu ; KO Validated
human	IHC	Cancer Lett; Ring finger protein 6 enhances chemo-resistance by transcriptionally activating proliferating cell nuclear antigen expression and attenuating DNA damage in lung adenocarcinoma.; Authors - Yile Sun
human	WB	Acta Pharmacol Sin; RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.; Authors - Ying Ren
human	WB	Anticancer Drugs; miR-203a suppresses cell proliferation by targeting RING-finger protein 6 in colorectal cancer.; Authors - Jiyu Miao
human	WB,IP	J Biol Chem; Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia; Authors - Haixia Zhuang
human	WB,IHC	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun