RNF8 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 14112-1-AP

Synonyms

KIAA0646, ring finger protein 8, RNF8

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Validation Data Gallery

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  WB analysis of HeLa using 14112-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14112-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14112-1-AP

  IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug.

• 

  IHC staining of human testis using 14112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14112-1-AP

  Immunofluorescent analysis of HeLa cells using 14112-1-AP (RNF8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

14112-1-AP targets RNF8 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF8 fusion protein Ag5292
• Full Name: ring finger protein 8
• Calculated Molecular Weight: 485 aa, 56 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC007517
• Gene Symbol: RNF8
• Gene ID (NCBI): 9025
• RRID: AB_2878016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

Publications

Species	Application	Title
mouse	IF,WB	Cell; Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.; Authors - Lan-Tao Gou
human	IP, IF	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human	WB	EMBO J; The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.; Authors - Abhay Narayan Singh ; KD Validated
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB,IF	EMBO J; BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.; Authors - Zhaohua Hu
human	WB,IF	EMBO Rep; 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.; Authors - Stavroula Tsaridou

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