Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11396-1-AP targets RPH3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1956 Product name: Recombinant human RPH3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC017259 Sequence: MTDTVFSNSSNRWMYPSDRPLQSKLQAGWSVHPGGQPDRQRKQEELTDEEKEIINRVIARAEKMEEMEQERIGRLVDRLENMRKNVAGDGVNRCILCGEQLGMLGSACVVCEDCKKNVCTKCGVETNNRLHSVWLCKICIEQREVWKRSGAWFFKGFPKQVLPQPMPIKKTKPQQPVSEPAAPEQPAPEPKHPARAPARGDSEDRRGPGQKTGPDPASAPGRGNYGPPVRRASEARMSSSSRDSESWDHSGGAGDSSRSPAGLRRANSVQASRPAPGSVQSPAPPQPGQPGTPGGSRPGP Predict reactive species |
| Full Name | rabphilin 3A homolog (mouse) |
| Calculated Molecular Weight | 690 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa |
| GenBank Accession Number | BC017259 |
| Gene Symbol | RPH3A |
| Gene ID (NCBI) | 22895 |
| RRID | AB_2181145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2J0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPH3A antibody 11396-1-AP | Download protocol |
| IHC protocol for RPH3A antibody 11396-1-AP | Download protocol |
| WB protocol for RPH3A antibody 11396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPH3A Polyclonal antibody (Cat# 11396-1-AP) has 8 total citations published in journals including Molecular Cell, Pharmacological Research, NPJ Parkinson's Disease, Cell Reports, British Journal of Pharmacology, Frontiers in Aging Neuroscience, Scientific Reports, and eLife. The associated research fields encompass synaptic transmission and neurotransmission, neurodegenerative diseases (Parkinson's disease, Alzheimer's disease, Huntington's disease), RNA modification, and exocytosis mechanisms.
| Species | Application | Title |
|---|---|---|
Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells | ||
Pharmacol Res Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease | ||
NPJ Parkinsons Dis Disruption of the LRRK2 substrate RAB12 facilitates neurotransmission and causes hyperactivity in mice. | ||
Cell Rep PCIF1-mediated m(6)Am modification in ACC neurons participates in inflammatory pain and anxiety. | ||
Br J Pharmacol Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats | ||
Front Aging Neurosci Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses |
