RPL7 Polyclonal antibody

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  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14583-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14583-1-AP

  IP result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug.

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  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 14583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPL7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14583-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

ribosomal protein L7, Large ribosomal subunit protein uL30, humL7 1, 60S ribosomal protein L7

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

14583-1-AP targets RPL7 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6104

  Product name: Recombinant human RPL7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-248 aa of BC006095

  Sequence: MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

• Full Name: ribosomal protein L7
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-30 kDa
• GenBank Accession Number: BC006095
• Gene Symbol: RPL7
• Gene ID (NCBI): 6129
• RRID: AB_2254049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18124
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	J Hematol Oncol; METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation; Authors - Meilin Xue
	WB	Nat Cell Biol; METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.; Authors - Rui Su
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB,RIP	Biochem Biophys Res Commun; CRISPR-based RNA-binding protein mapping in live cells.; Authors - Baiwen Chen
human	WB	Front Endocrinol (Lausanne); Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells; Authors - Manuel Bulfoni
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang