Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14583-1-AP targets RPL7 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPL7 fusion protein Ag6104|
|Full Name||ribosomal protein L7|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||29-30 kDa|
|GenBank accession number||BC006095|
|Gene ID (NCBI)||6129|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].
|Product Specific Protocols|
|WB protocol for RPL7 antibody 14583-1-AP||Download protocol|
|IHC protocol for RPL7 antibody 14583-1-AP||Download protocol|
|IF protocol for RPL7 antibody 14583-1-AP||Download protocol|
|IP protocol for RPL7 antibody 14583-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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