RPLP0 Monoclonal antibody

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• RPLP0 Monoclonal antibody (68056-1-Ig)

RPLP0 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68056-1-Ig Copied!

Clone No. 2A2E1

Publications (2)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, ELISA

Synonyms

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

• 

  WB analysis using 68056-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 68056-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 68056-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 68056-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, A549 cells, A375 cells, A431 cells, NCI-H1299 cells, PC-12 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68056-1-Ig targets RPLP0 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1829

  Product name: Recombinant human RPLP0 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-317 aa of BC009867

  Sequence: MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD

• Full Name: ribosomal protein, large, P0
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009867
• Gene Symbol: RPLP0
• Gene ID (NCBI): 6175
• RRID: AB_2918797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P05388
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPLP0 Monoclonal antibody (2A2E1), catalog number 68056-1-Ig, has 2 total citations. These appear in Biomolecules (2022) and Life Science Alliance (2024). The cited research spans neuroscience (alcohol-mediated neurocognitive dysfunction and brain insulin pathway signaling in rat models) and molecular cell biology (transcriptional regulators in TGFβ signaling in human samples). Both citations used Western blot as the application.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat		Biomolecules; Dietary Soy Prevents Alcohol-Mediated Neurocognitive Dysfunction and Associated Impairments in Brain Insulin Pathway Signaling in an Adolescent Rat Model.; Authors - Ming Tong
human	WB	Life Sci Alliance; Transcriptional regulators ensuring specific gene expression and decision-making at high TGFβ doses; Authors - Laura Hartmann