RPS3A Polyclonal antibody, PBS Only

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  WB analysis using 14123-1-AP (same clone as 14123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14123-1-AP (same clone as 14123-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 14123-1-AP (same clone as 14123-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 14123-1-AP (same clone as 14123-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14123-1-AP (same clone as 14123-1-PBS)

  IP result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:6000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14123-1-AP (same clone as 14123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14123-1-AP (RPS3A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (14123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human RPS3A (14123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 14123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL

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Product Information

14123-1-PBS targets RPS3A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5273

  Product name: Recombinant human RPS3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-264 aa of BC066926

  Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

• Full Name: ribosomal protein S3A
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC066926
• Gene Symbol: RPS3A
• Gene ID (NCBI): 6189
• RRID: AB_2253921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61247
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.