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RSK3 Polyclonal antibody

RSK3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 14446-1-AP

Synonyms

HU 2, MAPKAP kinase 1c, MAPKAPK 1c, MAPKAPK1C, p90 RSK 2, p90 RSK3, p90RSK2, pp90RSK3, Ribosomal S6 kinase 3, RPS6KA2, RSK, RSK 3, RSK3, S6K alpha, S6K alpha 2, S6K alpha2



Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14446-1-AP targets RSK3 in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSK3 fusion protein Ag5862
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 2
Calculated molecular weight 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC002363
Gene symbol RPS6KA2
Gene ID (NCBI) 6196
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RSK3, also named as RPS6KA2, belongs to the protein kinase superfamily. RSK3 is a serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. In addition RSK3 may function as tumor suppressor in epithelial ovarian cancer cells(Uniprot). The molecular weight of RSK3 is 83 kDa.

Protocols

Product Specific Protocols
WB protocol for RSK3 antibody 14446-1-APDownload protocol
IHC protocol for RSK3 antibody 14446-1-APDownload protocol
IF protocol for RSK3 antibody 14446-1-APDownload protocol
IP protocol for RSK3 antibody 14446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Epigenomics

Multiomics analysis identifies key genes and pathways related to N6-methyladenosine RNA modification in ovarian cancer.

Authors - Haoya Xu
humanWB

Theranostics

Kartogenin hydrolysis product 4-aminobiphenyl distributes to cartilage and mediates cartilage regeneration.

Authors - Shuai Zhang
mouseWB,IHC

Theranostics

RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells.

Authors - Shuai Zhang
  • KD Validated