RPUSD1 Polyclonal antibody, PBS Only

• 

  WB analysis using 25578-1-AP (same clone as 25578-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25578-1-AP (RPUSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

  IP result of anti-RPUSD1 (IP:25578-1-AP, 4ug; Detection:25578-1-AP 1:500) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPUSD1 antibody (25578-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

RPUSD1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 25578-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

RLUCL, C16orf40

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Product Information

25578-1-PBS targets RPUSD1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22314

  Product name: Recombinant human RPUSD1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-312 aa of BC011783

  Sequence: MEPGSVENLSIVYRSRDFLVVNKHWDVRIDSKAWRETLTLQKQLRYRFPELADPDTCYGFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVTISHAIGRNSTEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRIPTDTECVEVCTPDPFLPSLDACWSPHTLLQSLDQLVQALRATPDPDPEDRGPRPGSPSALLPGPGRPPPPPTKPPETEAQRGPCLQWLSEWTLEPDS

• Full Name: RNA pseudouridylate synthase domain containing 1
• Calculated Molecular Weight: 312 aa, 35 kDa
• Observed Molecular Weight: 34~35 kDa
• GenBank Accession Number: BC011783
• Gene Symbol: RPUSD1
• Gene ID (NCBI): 113000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJJ7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949).