RRAGD Polyclonal antibody, PBS Only

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  WB analysis using 15315-1-AP (same clone as 15315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 15315-1-AP (same clone as 15315-1-PBS)

  IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15315-1-AP (same clone as 15315-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRAGD antibody (15315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

RRAGD Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 15315-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.6.5.-, Rag D, RagD, Ras related GTP binding D, Ras-related GTP-binding protein D

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Product Information

15315-1-PBS targets RRAGD in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7557

  Product name: Recombinant human RRAGD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-249 aa of BC003088

  Sequence: MEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL

• Full Name: Ras-related GTP binding D
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC003088
• Gene Symbol: RRAGD
• Gene ID (NCBI): 58528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQL2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.