RUFY1 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa cells using 13498-1-AP (same clone as 13498-1-PBS)

  IP result of anti-RUFY1 (13498-1-AP for IP and Detection). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

  Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

RUFY1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 13498-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

13498-1-PBS targets RUFY1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUFY1 fusion protein Ag4324
• Full Name: RUN and FYVE domain containing 1
• Calculated Molecular Weight: 708 aa, 80 kDa
• Observed Molecular Weight: 69 kDa, 80 kDa
• GenBank Accession Number: BC032571
• Gene Symbol: RUFY1
• Gene ID (NCBI): 80230
• RRID: AB_2183747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T51
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.