RUFY1 Polyclonal antibody

RUFY1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13498-1-AP

Synonyms

FYVE finger protein EIP1, La binding protein 1, Rab4 interacting protein, RABIP4, RUFY1, ZFYVE12

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Validation Data Gallery

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  WB analysis of HeLa using 13498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 13498-1-AP

  IP result of anti-RUFY1 (13498-1-AP for IP and Detection).

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  IF Staining of HeLa using 13498-1-AP

  Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

13498-1-AP targets RUFY1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUFY1 fusion protein Ag4324
• Full Name: RUN and FYVE domain containing 1
• Calculated Molecular Weight: 708 aa, 80 kDa
• Observed Molecular Weight: 69 kDa, 80 kDa
• GenBank Accession Number: BC032571
• Gene Symbol: RUFY1
• Gene ID (NCBI): 80230
• RRID: AB_2183747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

Publications

Species	Application	Title
human	IF	Nat Commun; Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.; Authors - Meng Shi
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS
human	WB, IF	Mol Biol Cell; COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.; Authors - Christine A Phillips-Krawczak
human	CoIP	Cancer Sci; Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.; Authors - Qiaoming Zhi