RUFY1 Polyclonal antibody

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• RUFY1 Polyclonal antibody (13498-1-AP)

RUFY1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13498-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

RUN and FYVE domain-containing protein 1, RUFY 1, RABIP4, Rab4-interacting protein, La-binding protein 1

• 

  WB analysis of HeLa using 13498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa cells using 13498-1-AP

  IP result of anti-RUFY1 (13498-1-AP for IP and Detection).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 13498-1-AP

  Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13498-1-AP targets RUFY1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4324

  Product name: Recombinant human RUFY1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 219-600 aa of BC032571

  Sequence: TKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

• Full Name: RUN and FYVE domain containing 1
• Calculated Molecular Weight: 708 aa, 80 kDa
• Observed Molecular Weight: 69 kDa, 80 kDa
• GenBank Accession Number: BC032571
• Gene Symbol: RUFY1
• Gene ID (NCBI): 80230
• RRID: AB_2183747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T51
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

Publications

What published studies show

The RUFY1 Polyclonal antibody (Cat# 13498-1-AP) has 8 citations published in Cell, Nature Communications, Advanced Science, Nature Structural & Molecular Biology, EMBO Journal, Cancer Science, and Molecular Biology of the Cell. Research fields include endosomal and vesicular trafficking, oocyte maturation and female fertility, proteostasis, gastric cancer progression, and copper transporter regulation.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Cell; Mouse oocytes sequester aggregated proteins in degradative super-organelles; Authors - Gabriele Zaffagnini
human	IF	Nat Commun; Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.; Authors - Meng Shi
mouse	IF	Adv Sci (Weinh); CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes.; Authors - Ying Tian
mouse	IF	Nat Struct Mol Biol; The maternal PADI6-UHRF1-UBE2D complex regulates ubiquitination during oocyte maturation and embryogenesis.; Authors - Jinhong Li
mouse	IF	EMBO J; 20S proteasome-regulated proteostasis in ELVAs is critical for oocyte-to-embryo transition and female fertility.; Authors - Yan Rong
human	CoIP	Cancer Sci; Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.; Authors - Qiaoming Zhi