RUNDC3A Polyclonal antibody

• Featured Product
• KD/KO Validated

RUNDC3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20531-1-AP

Synonyms

Rap2 interacting protein 8, RAP2IP, RPIP 8, RPIP8, RUN domain containing 3A, RUNDC3A

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Validation Data Gallery

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  WB analysis using 20531-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20531-1-AP (RUNDC3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20531-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20531-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20531-1-AP targets RUNDC3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUNDC3A fusion protein Ag14393
• Full Name: RUN domain containing 3A
• Calculated Molecular Weight: 446 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC006194
• Gene Symbol: RUNDC3A
• Gene ID (NCBI): 10900
• RRID: AB_10695757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).; Authors - Pengchen Chen ; KO Validated