RXFP1 Polyclonal antibody

RXFP1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18419-1-AP

Synonyms

Relaxin receptor 1, LGR7.2, LGR7.10, LGR7.1, LGR7

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Validation Data Gallery

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  IHC staining of human brain using 18419-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18419-1-AP (RXFP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18419-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18419-1-AP (RXFP1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18419-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18419-1-AP (RXFP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18419-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18419-1-AP (RXFP1 Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse testis using 18419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RXFP1 antibody (18419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 18419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RXFP1 antibody (18419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 18419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using RXFP1 antibody (18419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue, mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

18419-1-AP targets RXFP1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RXFP1 fusion protein Ag13098
• Full Name: relaxin/insulin-like family peptide receptor 1
• Calculated Molecular Weight: 757 aa, 87 kDa
• GenBank Accession Number: BC112142
• Gene Symbol: RXFP1
• Gene ID (NCBI): 59350
• RRID: AB_2878543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC	Bone Rep; Relaxin 2 carried by magnetically directed liposomes accelerates rat midpalatal suture expansion and subsequent new bone formation.; Authors - Hiroyuki Kamimoto