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Recombinant Rat CD3 epsilon protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1450

Validation Data Gallery

  • Purity of Recombinant Rat CD3 epsilon was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat CD3 epsilon was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CD3 epsilon protein Ile24-Tyr103 (Accession# NP_001101610.2) with a rabbit IgG Fc tag at the C-terminus.
GeneID 315609
Accession NP_001101610.2
PredictedSize 35.5 kDa
SDS-PAGE 38-48 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. CD3 is composed of CD3γ, δ, ε, and ζ chains. It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction This polyclonal antibody was raised against the epsilon chain of rat CD3 molecule.

References:

1. Yang H. et al. (2005). Immunology. 115(2):189-96.
2. Clevers H. et al. (1988). Annu Rev Immunol. 6:629-62.
3. Cantrell DA. (2002). Immunology. 105(4):369-74.