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Recombinant Rat GM-CSF protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

N-rFc

Activity

not tested

Cat no : Eg3202

Validation Data Gallery

  • Purity of Recombinant Rat GM-CSF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat GM-CSF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat GM-CSF protein Ala18-Lys144 (Accession# P48750) with a rabbit IgG Fc tag at the N-terminus.
GeneID 116630
Accession P48750
PredictedSize 41.7 kDa
SDS-PAGE 42-50 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

GM-CSF (also known as CSF2) is a member of the CSF family of hematopoietic growth factors and was originally identified as a stimulus that induces the differentiation of bone marrow precursor cells into granulocytes and macrophages. GM-CSF is secreted as monomeric glycoprotein. Multiple cellular sources of GM-CSF have been described, including epithelial cells, endothelial cells, fibroblasts, stromal cells, and hematopoietic cells. GM-CSF not only stimulates granulocyte proliferation and differentiation but also promotes vasculogenesis via chemotactic macrophages and neutrophil generation. GM-CSF has a wide range of biological activities in innate and adaptive immunity, and it plays a key role in many autoimmune and inflammatory diseases.

References:

1.Becher B. et al. (2016) Immunity. 45(5):963-973.
2.Ingelfinger F. et al. (2021) Semin Immunol. 54:101518.
3.Chen Y. et al. (2023) Front Immunol. 14:1158859. 
4.Wei W. et al. (2023) Mol Hum Reprod. 30(1):gaad042.