Recombinant Rat IFN-gamma R1 protein (hFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

not tested

Cat no : Eg0961

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Validation Data Gallery

• 

  Purity of Recombinant Rat IFN-gamma R1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat IFN-gamma R1 protein Ala17-Ser241 (Accession# Q9QZ62) with a human IgG1 Fc tag at the C-terminus.
• GeneID: 116465
• Accession: Q9QZ62
• PredictedSize: 52.2 kDa
• SDS-PAGE: 55-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection.

References:

1.Der SD. et al.(1998) Proc Natl Acad Sci. 95(26):15623-8.
2.Michalska A. et al.(2018) Front Immunol. 9:1135.
3.Gocher AM. et al.(2022) Nat Rev Immunol. 22(3):158-72.
4.Schoenborn, JR. et al.(2007) Adv immunol. 96:41-101.
5.Larkin, J.et al.(2000) J interf cytok res. 20(6):565-76.