Recombinant Rat IL-10 protein (His Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0269
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat IL-10 protein Ser19-Asn178 (Accession# P29456) with a his tag at the C-terminus. |
| GeneID | 25325 |
| Accession | P29456 |
| PredictedSize | 19.8 kDa |
| SDS-PAGE | 26-30 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin 10 (IL-10) is an essential anti-inflammatory cytokine that plays important roles as a negative regulator of immune responses to microbial antigens. IL-10 is mainly secreted by macrophages, but also by T helper 1 (Th1) and Th2 lymphocytes, dendritic cells, cytotoxic T cells, B lymphocytes, monocytes and mast cells. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10 activity is mediated by the IL-10 receptor (IL-10R) which is a member of the class II cytokine receptor family.
References:
1. Pisa P. et al. (1992) Proc Natl Acad Sci U S A. 89(16):7708-12. 2. Gastl GA. et al. (1993) Int J Cancer. 55(1):96-101. 3. Knolle PA. et al. (1998) Clin Exp Immunol. 114(3):427-33. 4. Heine G. et al. (2019) Eur J Immunol. 44(6):1615-21. 5. Shouval DS. et al. (2014) Adv Immunol. 122:177-210.