Recombinant Rat M-CSF protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3414

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Validation Data Gallery

• 

  Purity of Recombinant Rat M-CSF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat M-CSF protein Glu33-Pro186 (Accession# Q8JZQ0-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 78965
• Accession: Q8JZQ0-1
• PredictedSize: 44.3 kDa
• SDS-PAGE: 46-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

M-CSF, also called colony-stimulating factor-1 or CSF-1), is a haematopoietic growth factor, which controls survival, proliferation, differentiation and function of mononuclear phagocytes. It also plays a role in bone metabolism, fertility, pregnancy and inflammatory processes. M-CSF may be expressed on the surface of the cell as a membrane-spanning glycoprotein or secreted into circumstance. M-CSF was identified as an 84-kDa homodimeric glycoprotein found in healthy human urine, and is present in the peripheral blood and urine. M-CSF serum levels were elevated in pre-transplant haemodialysis patients, so it might be a specific marker of Kidney Allograft Rejection.

References:

1. J W Pollard, et al. (1987). Nature. 330(6147): 484-486.
2. B R Witt, et al. (1997). Fertil Steril. 68(2): 259-264.
3. Anna Popova, et al. (2010). Immunobiology. 216(1-2):164-172.
4. K Motoyoshi, et al. (1998). Int J Hematol.67(2):109-122.
5. K Motoyoshi, et al. (1978). Blood. 52(5): 1012-1020.
6. Yannick Le Meur, et al. (2004). Nephrol Dial Transplant.19(7):1862-1865.