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Recombinant Rat Osteopontin protein (C-rFc)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3247

Validation Data Gallery

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <1.0 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat Osteopontin protein Leu17-Asn317 (Accession# P087211) with a rabbit IgG Fc tag at the C-terminus.
GeneID 25353
Accession P08721
PredictedSize 59.5 kDa
SDS-PAGE
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Osteopontin (OPN), also called secreted phosphoprotein 1 (SPP1), is encoded by the gene SPP1. OPN is a glycoprotein and presents different post-translational modifications according to the tissue of expression. OPN is a multifunctional phosphorylated protein involved in physiological and pathological events. Under physiological conditions, circulating and tissue OPN expression levels are relatively low, and OPN is suggested to play a key role in regulating biomineralization, wound healing, and developmental processes. Under pathological conditions, OPN is often significantly upregulated and exerts pleiotropic effects in several inflammatory, autoimmune, degenerative, fibrosis, and oncologic diseases, such as diabetes, stroke, kidney injury, cardiac fibrosis, and cancers.

References:

1.Lamort AS. et al. (2019) Cells. 8(8):815.
2.Shirakawa K and Sano M. (2021) Biomolecules. 11(7):1047.
3.Yim A. et al. (2022) Immunol Rev. 311(1):224-233.
4.Lin EY. et al. (2023) Int Immunol. 35(4):171-180.
5.Jia Q. et al. (2024) Lung. 202(1):25-39.