Recombinant Rat Serpin E1/PAI-1 protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3256

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Validation Data Gallery

• 

  Purity of Recombinant Rat PAI-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat PAI-1 protein Ser24-Pro402 (Accession# P20961) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 24617
• Accession: P20961
• PredictedSize: 68.9 kDa
• SDS-PAGE: 70-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

PAI-1 (Plasminogen Activator Inhibitor-1), also known as Serpin E1, is a member of the serine protease inhibitor (SERPIN) family. PAI-1, a single-chain glycoprotein of approximately 52 kDa, acts as an inhibitor of tissue plasminogen activator and urokinase-type plasminogen activator and affects fibrinolysis and plays an important role in lung diseases. Because PAI-1 may display cell type-specific glycosylation and activity, the glycosylation pattern of PAI-1 may serve as a potential biomarker for predicting cellular dysfunction and risk of thrombosis. Recent studies have revealed that PAI-1 has a pro-tumorigenic role in human cancers by regulating angiogenesis and tumor cell survival. It is suggested that increased vascular PAI-1 can directly accelerate the process of atherosclerotic thrombosis by promoting the formation of neointimal plaques.

References:

1. Altalhi R, et al. (2021) Int J Mol Sci. 22(6):3170.                                                     
2. Ali G, et al. (2023) Stem Cell Res Ther. 14(1):185.                                                    
3. Furuya H, et al. (2022) Sci Rep. 12(1):12186.