Recombinant Rat Progranulin/PGRN protein (mFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg3240

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Validation Data Gallery

• 

  Purity of Recombinant Rat Progranulin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat Progranulin protein Thr18-Leu588 (Accession# P23785) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 29143
• Accession: P23785
• PredictedSize: 88.1 kDa
• SDS-PAGE: 85-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, was first identified as a 593-aa secreted glycoprotein involved in the regulation of cancer progression and wound healing. It is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin (PGRN) has been considered a growth factor-like molecule implicated in additional biological and pathological processes including early embryogenesis, inflammation, host defense, cartilage development and degradation.

References:

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4. Zhiheng He. et al. (2003). Nat Med. 9(2):225-229.  
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7. L Díaz-Cueto. et al. (2000). Dev Biol. 217(2):406-418.