Recombinant Rat SCF protein (His Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1092

---

Validation Data Gallery

• 

  Purity of Recombinant Rat SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat SCF protein Gln26-Ala189 (Accession# P21581-1) with a His tag at the C-terminus.
• GeneID: 60427
• Accession: P21581-1
• PredictedSize: 19.4 kDa
• SDS-PAGE: 20-33 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

References:

1. Gu Y, et al, (2011) PLoS One. 6(10):e25984.
2. Pedersen M, et al. (2008) Biochem Biophys Res Commun. 377(1):98-103.
3. Talaiezadeh A, et al. (2012) Contemp Oncol (Pozn). 16(4):306-9.
4. Bai CG, et al. (2012) World J Gastroenterol.18(23):2929-37.
5. Lennartsson J, et al. (2012) Physiol Rev. 92(4):1619-49.
6. Liang J, et al. (2013) Int J Biol Sci. 9(5):435-43.