S100A7/Psoriasin Polyclonal antibody

S100A7/Psoriasin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26656-1-AP

Synonyms

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

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Validation Data Gallery

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  WB analysis of MCF-7 using 26656-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-468 using 26656-1-AP

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 26656-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 26656-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-468 cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26656-1-AP targets S100A7/Psoriasin in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A7/Psoriasin fusion protein Ag24717
• Full Name: S100 calcium binding protein A7
• Calculated Molecular Weight: 101 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC034687
• Gene Symbol: S100A7
• Gene ID (NCBI): 6278
• RRID: AB_2880592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.