Anti-Mouse S100A8 Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98467-2-RR

  1x10^6 HEK-293T cells (left) or S100A8-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse S100A8 Rabbit RecAb (98467-2-RR, Clone: 250113D8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

S100A8 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98467-2-RR

Clone No.250113D8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98467-2-RR targets S100A8 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S100A8 fusion protein Eg2959
• Full Name: S100 calcium binding protein A8 (calgranulin A)
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: NM_013650.2
• Gene Symbol: S100a8
• Gene ID (NCBI): 20201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27005
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.