SALL4 Polyclonal antibody

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 24500-1-AP

  IP result of anti-SALL4 (IP:24500-1-AP, 4ug; Detection:24500-1-AP 1:600) with HepG2 cells lysate 3600ug.

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IHC staining of mouse testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24500-1-AP (SALL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IF Staining of Caco-2 using 24500-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (24500-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

SALL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24500-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Zinc finger protein SALL4, Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse testis tissue, human ovary tumor tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

24500-1-AP targets SALL4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SALL4 fusion protein Ag17480
• Full Name: sal-like 4 (Drosophila)
• Calculated Molecular Weight: 1053 aa, 112 kDa
• Observed Molecular Weight: 66-75 kDa
• GenBank Accession Number: BC111714
• Gene Symbol: SALL4
• Gene ID (NCBI): 57167
• RRID: AB_2879576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJQ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sal-like protein 4 (SALL4), also named Zinc finger protein 797, contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is a transcription factor that exists in two splice isoforms and regulates transcription in embryonic stem cells, hematopoiesis, and acute myeloid leukemia. SALL4 is activated in fetal liver tissue, silenced in adult liver tissue, and reexpressed as an oncofetal protein in hepatocellular carcinoma (PMID: 26892498, 23758232). SALL4 has two isoforms with predicted molecular weights of 112 kDa (SALL4A) and 66 kDa (unmodified SALL4B), and SALL4B was found to be the dominant endogenous isoform in HCC cell lines. Ectopically expressed SALL4B migrated at 95 kDa modified by ubiquitination, 130 kDa modified by sumoylation (PMID: 23012367).

Publications

Species	Application	Title
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	IHC,WB	Cell Signal; miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.; Authors - Dong-Min Shi
human	WB	Onco Targets Ther; PMID: 31632072; Authors - Chiung-Chi Cheng
human	WB	Sci Rep; MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.; Authors - Yancheng Lin
human	WB	Pathol Int; Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1; Authors - Qing-Dong Wang
human	WB,IHC,IF	J Mol Histol; Expression and clinical significance of SALL4 and β-catenin in colorectal cancer.; Authors - Liliang Hao