Validation Data Gallery
|Positive WB detected in||HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human testis tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24500-1-AP is SALL4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SALL4 fusion protein Ag17480|
|Full Name||sal-like 4 (Drosophila)|
|Calculated molecular weight||1053 aa, 112 kDa|
|Observed molecular weight||66-75 kDa|
|GenBank accession number||BC111714|
|Gene ID (NCBI)||57167|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.
|Product Specific Protocols|
|WB protocol for SALL4 antibody 24500-1-AP||Download protocol|
|IHC protocol for SALL4 antibody 24500-1-AP||Download protocol|
|IF protocol for SALL4 antibody 24500-1-AP||Download protocol|
|IP protocol for SALL4 antibody 24500-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.
miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.
Onco Targets Ther
MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.
Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1
J Mol Histol
Expression and clinical significance of SALL4 and β-catenin in colorectal cancer.