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  • KD/KO Validated

SAMHD1 Polyclonal antibody

SAMHD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12586-1-AP

Synonyms

DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inTHP-1 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12586-1-AP targets SAMHD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMHD1 fusion protein Ag3287
Full Name SAM domain and HD domain 1
Calculated molecular weight 626 aa, 72 kDa
Observed molecular weight 64-72 kDa
GenBank accession numberBC036450
Gene symbol SAMHD1
Gene ID (NCBI) 25939
RRIDAB_2183496
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for SAMHD1 antibody 12586-1-APDownload protocol
IHC protocol for SAMHD1 antibody 12586-1-APDownload protocol
IF protocol for SAMHD1 antibody 12586-1-APDownload protocol
IP protocol for SAMHD1 antibody 12586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Genet

Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.

Authors - Rice Gillian I GI
humanIF

Nat Microbiol

Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.

Authors - Ramona Businger
humanWB,IHC

Nat Med

PMID: 27991919

Authors - Constanze Schneider
humanWB,IHC

Nat Med

PMID: 28067901

Authors - Nikolas Herold
humanWB, IHC, IF

Nat Med

SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.

Authors - Baldauf Hanna-Mari HM
  • KD Validated
monkeyWB

Immunity

Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.

Authors - Nina Calantone