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- KD/KO Validated
SAMHD1 Polyclonal antibody
SAMHD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12586-1-AP
Validation Data Gallery
|Positive WB detected in
|HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|THP-1 cells, K-562 cells
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12586-1-AP targets SAMHD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, monkey
|Host / Isotype
|Rabbit / IgG
|SAMHD1 fusion protein Ag3287
|SAM domain and HD domain 1
|Calculated molecular weight
|626 aa, 72 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
|Product Specific Protocols
|WB protocol for SAMHD1 antibody 12586-1-AP
|IHC protocol for SAMHD1 antibody 12586-1-AP
|IF protocol for SAMHD1 antibody 12586-1-AP
|IP protocol for SAMHD1 antibody 12586-1-AP
|Click here to view our Standard Protocols
Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.
Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.
SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.
Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.