SAP 97 Polyclonal antibody

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  WB analysis of mouse lung using 55085-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55085-1-AP (DLG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55085-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55085-1-AP (DLG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SAP 97 Polyclonal Antibody for WB, ELISA

Cat No. 55085-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

Disks large homolog 1, DLG1, DLGH1, hdlg, SAP 97, SAP97, Synapse associated protein 97

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Tested Applications

• Positive WB detected in: mouse lung tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

55085-1-AP targets SAP 97 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: discs, large homolog 1 (Drosophila)
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 120 kDa, 130-140 kDa
• GenBank Accession Number: NM_004087
• Gene Symbol: SAP97
• Gene ID (NCBI): 1739
• RRID: AB_11182375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12959
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse, human	IF	Kidney Int; Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.; Authors - Rik Westland
rat	WB	Neurochem Res; PFOS Elicits Cytotoxicity in Neuron Through Astrocyte-Derived CaMKII-DLG1 Signaling In Vitro Rat Hippocampal Model; Authors - Jiawei Yang